Figure 2.

Anaerobic transcription of PaiI requires NarXL. (A) Expression of PaiI in PA14 B-96 cells. Total RNA was extracted from planktonically growing cells (P) and from anoxic biofilms (B-96) under the same conditions as described (Tata et al., 2016) and the PaiI was detected as described in Materials and Methods. (B) The NarXL two-component regulatory system is required for expression of PaiI. Detection of PaiI by Northern-blotting in total RNA extracted from PAO1 and PA14, and different mutants thereof grown in anoxic biofilms (B-96). 0.6 ng of in vitro transcribed PaiI (126 nt) was loaded as a control (C).