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. 2017 Nov 27;7:16407. doi: 10.1038/s41598-017-16759-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Low dose treatment of SKOV3 with miuraenamide A (Miu) showed no effects on cell viability, proliferation and actin cytoskeleton morphology. (a) Cell viability after treatment for 72 h of SKOV3. (b) PI exclusion assay after treatment for 72 h with 20 nM Miu. (c) Cell cycle analysis. (d) Proliferation after treatment with increasing concentrations of Miu. (e) Actin staining of SKOV3 cells treated with 20 nM Miu for the indicated time points (rhodamine-phalloidin, red, and nucleus, Hoechst 33342, blue). White bars: 10 µm. ns, not significant. One-Way ANOVA/Bonferroni’s Multiple Comparison Test, *p < 0.05. n = 3.