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. 2017 Nov 27;7:16407. doi: 10.1038/s41598-017-16759-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of SKOV3 cell migration. SKOV3 cells were pre-treated with 20 nM Miu over 72 h, during the migration process no compound was present. (a) Boyden chamber migration assay over 24 h migration through a 8 µm pore membrane. Paired two tailed t-test, *p < 0.05. (b) Wound healing assay, neg Ctrl: negative control, migration in FCS free medium. One representative image per treatment is shown. (c) Chemotaxis assay over 24 h along an FCS gradient. FMI, forward migration index, +/+ Ctrl: positive control, medium with FCS on both sides, no gradient. One-Way ANOVA/Bonferroni’s Multiple Comparison Test, *p < 0.05. n = 3.