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. 2017 Nov 27;7:16407. doi: 10.1038/s41598-017-16759-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of migration associated parameters. (a) Adhesion and (b) Spreading of SKOV3 cells pre-treated with 20 nM Miu over 72 h were quantified after 90 min by counting adhered or spread cells. (c) HRP secretion assay with HeLa-ssHRP. Brefeldin A (BFA) was used as positive control, fresh Miu was added ( + 4 h) or not (−4 h) during secretion, n = 4. One-Way ANOVA/Bonferroni’s Multiple Comparison Test, *p < 0.05.