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. 2017 Nov 27;7:16444. doi: 10.1038/s41598-017-16764-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of TERT promoter activities during ESC differentiation. (A) TERT promoter activities in differentiating EB cultures. Cells were harvested for luciferase assays following the initiation of differentiation. (B) TERT promoter activities in differentiated osteogenic and fibroblast-like cells. Luciferase activities were measured in extracts from undifferentiated ESCs and their differentiated derivatives. P3, passage 3; P3-RA, P3 cells that were treated with 0.15 µM retinoic acid for two days. (C) Images of in vitro differentiated osteogenic and RA-treated cells. a, osteogenic cells were stained by Alizarin red. Differentiated cells were stained with an anti-vimentin antibody in right panels: b, bright field; c, vimentin antibody staining; d, DAPI staining; e, merged image.