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. 2017 Nov 27;7:16444. doi: 10.1038/s41598-017-16764-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chromatin structures of TERT genes in ESCs and differentiated cells. (A) A schematic diagram of the genomic region spanning the TERT promoters in the BAC reporters. Positions of qPCR amplicons are labeled as: A, −5 kb; B, −2 kb; P, TERT promoter; R, Rluc ORF (approximately + 1 kb downstream of the TERT promoters in the BAC reporters); C, + 1 kb; and D, +2 kb. The positions are relative to the TERT TSSs in the native genomic sequence (without Rluc ORF). Italic/underlined labels indicate corresponding mouse amplicons. (B) Covalent histone modifications at the TERT promoters. Chromatin fragments from ESCs containing the BAC reporters were precipitated using antibodies against specific histone marks, followed by qPCR analyses. H4Ac refers to acetylated histone H4. H3K4me3, H3K9me3, and H3K27me3 are trimethylated K4, K9, and K27 residues of histone H3. Open circles and closed triangles are undifferentiated ESCs and differentiated fibroblast-like cells, respectively.