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. 2017 Nov 27;7:16335. doi: 10.1038/s41598-017-16451-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antiviral activity of αRep4E3 and αRep9A8. (a), PR-mediated maturation cleavage of Pr55Gag. Control cells (unmodified SupT1 and SupT1/αRep9C2-GFP), and αRep-expressing cells (SupT1/αRep4E3-GFP and SupT1/αRep9A8-GFP) were infected with HIV-1 at the same MOI, and extracellular media collected. After normalization to the CAp24 level (80 µg per sample), the degree of proteolytic cleavage of Pr55Gag at the MA-CA junction by the viral protease (PR) was evaluated using an ELISA-based maturation assay. The bar graph represents the percentage of cleaved Pr55Gag (mean ± SD; n = 3), estimated from the degree of accessibility and reactivity of a Gag-embedded MA epitope towards its specific monoclonal antibody, in competition with a free synthetic peptide reproducing its sequence (refer to Materials & Methods). (b), Viral genome packaging. Bar graph representing the concentrations of HIV-1 genomes in culture media after normalization to CAp24 levels (genome copy number per 25 µg CAp24; mean ± SD; n = 3), and the fold changes in culture media of SupT1/αRep4E3-GFP and SupT1/αRep9A8-GFP cells, compared to control cell lines SupT1 and SupT1/αRep9C2-GFP. (**), P < 0.01; (*), P < 0.05; ns, not significant.