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. 2017 Nov 27;7:16335. doi: 10.1038/s41598-017-16451-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Infectivity of virus progeny released by HIV-1-infected, αRep-expressing SupT1 cells. Control cells (unmodified SupT1 and SupT1/αRep9C2-GFP), and αRep-expressing cells (SupT1/αRep4E3-GFP and SupT1/αRep9A8-GFP) were infected with HIV-1 at the same MOI. Samples from culture media were collected at D14 pi, and re-inoculated to Tat-dependent, indicator T-cells (Jurkat-GFP cells) after adjustment of the CAp24 levels to 15 µg per sample. Jurkat-GFP cells were harvested at D10 and D14 post-reinfection, and GFP-positive cells determined by flow cytometry. The results presented in the bar graph (mean ± SD; n = 3) are the percentages of GFP-positive, fluorescent cells. (**), P < 0.01; ns, not significant.