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. 2017 Nov 27;7:16335. doi: 10.1038/s41598-017-16451-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intravesicular assembly of VLPs in αRep9A8-expressing insect cells. Electron micrographs of ultrathin sections of AcMNPV^gag-infected Sf/(Myr+)αRep9A8-GFP cells at 48hrs post-infection. The cell presented in panel (a) shows the abnormal accumulation of Pr55Gag polyprotein at the plasma membrane, and the occurrence of VLPs within intracytoplasmic vesicles. Panels (b,c) present different cell sections at high magnification, showing simultaneous intravesicular budding and plasma membrane budding of VLPs. IC, intercellular space; EC, extracellular space; PM, plasma membrane; Cy, cytoplasm; IV, intracellular vesicle; Nu, nucleoplasm; NM, nuclear membrane.