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. 2017 Nov 27;7:16374. doi: 10.1038/s41598-017-12562-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Baicalein blocks LPS-induced MD-2/TLR4 association. RAW264.7 cells were pretreated with baicalein (0, 10, 25, 50 μM) for 2 h and then stimulated with LPS (1 μg/ml) for 24 h. Cell lysates were immunoprecipitated with anti-TLR4 antibody and then subjected to immunoblotting analysis of MD-2 and TLR4. Densitometric analysis of the MD-2/TLR4 ratio in the immunoblots of the immunoprecipitates was carried out. ^###p < 0.001 vs. the mock group; **p < 0.01, ***P < 0.001 vs. the LPS group.