Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 27;7:16422. doi: 10.1038/s41598-017-16616-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CCND1 and CCNE1 facilitate EV71 replication. (a) The expression of CCND1 and CCNE1 were induced by EV71 infection. SK-N-SH cells were harvested after EV71 infection for the indicated times, and the expression levels of CCND1 and CCNE1 were measured by qRT-PCR. (b,c) Overexpression CCND1 and CCNE1 promote EV71 replication. SK-N-SH cells were transfected with Flag-CCND1, CCNE1-Flag and empty vector PCAGGS. 24 h after transfection, the cells were infected with EV71 for 15 h and then harvested for qRT-PCR (b) and western blot (c) assays. (d,e) Knock-down of the expression levels of CCND1 and CCNE1 attenuates EV71 replication. SK-N-SH cells were transfected with control siRNA (NC) or individual-specific siRNAs directed against CCND1 or CCNE1. Cells were infected with EV71 for 15 h at 48 h after transfection. Then, the cells were assayed for expression of EV71 genes, CCND1 and CCNE1 by qRT-PCR (d) and western blot assay (e). Data in panels (a,b and d) are representative of at least three independent experiments, with each determination performed in triplicate (mean ± SD). Asterisks denote significant differences between indicated samples (*p < 0.05, **p < 0.01, Student’s t test). The blots in c and e were cropped and the full-length blots were displayed in Supplementary Information file.