Table 2.
List of significant QTL detected in four environments for flag leaf length (FLL), flag leaf width (FLW) and flag leaf area (FLA) using high-density linkage map.
| Trait | Env.1 | QTL | Chr.2 | Pos3 | Flanking markers | LOD | PVE4 | Add5 |
|---|---|---|---|---|---|---|---|---|
| FLL | L15 | Qfll.hww-2D.a | 2D | 0 | HWGBS1994-HWGBS1859 | 4.00 | 9.65 | 3.51 |
| Qfll.hww-2D.b | 2D | 58 | HWGBS1918-HWGBS1863 | 4.77 | 9.59 | 3.56 | ||
| Qfll.hww.6A | 6A | 64 | HWGBS3922-HWGBS3920 | 3.77 | 9.32 | 3.49 | ||
| Qfll.hww.7A | 7A | 78 | HWGBS5487-HWGBS5600 | 5.91 | 14.22 | 3.64 | ||
| L16 | Qfll.hww-2D.c | 2D | 13 | HWGBS1948-HWGBS1887 | 3.74 | 8.91 | 3.52 | |
| Qfll.hww-7A | 7A | 78 | HWGBS5487-HWGBS5600 | 3.61 | 9.50 | 3.51 | ||
| Qfll.hww-7B | 7B | 24 | HWGBS5671-HWGBS5669 | 3.30 | 8.72 | 3.48 | ||
| M15 | Qfll.hww-5A | 5A | 28 | HWGBS3329-HWGBS3337 | 3.74 | 7.93 | −3.34 | |
| Qfll.hww-7A | 7A | 78 | HWGBS5487-HWGBS5600 | 3.64 | 7.83 | 3.34 | ||
| M16 | Qfll.hww-2D.c | 2D | 13 | HWGBS1948-HWGBS1887 | 3.76 | 8.46 | 3.3 | |
| Qfll.hww-2D.b | 2D | 58 | HWGBS1918-HWGBS1863 | 3.84 | 8.66 | 3.31 | ||
| Qfll.hww-6A | 6A | 64 | HWGBS3922-HWGBS3920 | 4.49 | 9.55 | 3.33 | ||
| Qfll.hww-7A.b | 7A | 75 | HWGBS5458-HWGBS5471 | 4.98 | 10.62 | 3.35 | ||
| FLW | L15 | Qflw.hww-5A | 5A | 28 | HWGBS3329-HWGBS3337 | 3.72 | 10.28 | −1.01 |
| L16 | Qflw.hww-1A | 1A | 111 | HWGBS136-HWGBS115 | 3.90 | 9.97 | −1.03 | |
| Qflw.hww-5A | 5A | 28 | HWGBS3329-HWGBS3337 | 4.71 | 10.94 | −1.03 | ||
| M15 | Qflw.hww-1B | 1B | 66 | HWGBS799-HWGBS805 | 14.54 | 16.53 | 1.04 | |
| Qflw.hww-5A | 5A | 28 | HWGBS3329-HWGBS3337 | 5.55 | 6.95 | −1.02 | ||
| Qflw.hww-7A | 7A | 87 | HWGBS5576-HWGBS5480 | 3.61 | 5.21 | 1.02 | ||
| M16 | Qflw.hww-5A | 5A | 28 | HWGBS3329-HWGBS3337 | 3.96 | 10.86 | −1.04 | |
| FLA | L15 | Qfla.hww-2D.a | 2D | 63 | HWGBS1860-HWGBS1994 | 4.39 | 11.02 | 3.73 |
| Qfla.hww-7A | 7A | 78 | HWGBS5487-HWGBS5600 | 5.83 | 14.57 | 3.86 | ||
| L16 | Qfla.hww-2D.b | 2D | 30 | HWGBS1929-HWGBS1851 | 3.50 | 8.28 | 3.95 | |
| Qfla.hww-3B | 3B | 182 | HWGBS2422-HWGBS2280 | 3.51 | 8.32 | −3.96 | ||
| Qfla.hww-4B | 4B | 32 | HWGBS3140-HWGBS3186 | 3.74 | 8.23 | 3.95 | ||
| Qfla.hww-6A | 6A | 72 | HWGBS3975-HWGBS3938 | 3.28 | 7.59 | 3.9 | ||
| Qfla.hww-7D | 7D | 63 | HWGBS6027-HWGBS6033 | 3.24 | 7.20 | −3.87 | ||
| M15 | Qfla.hww-3A | 3A | 0 | HWGBS2032-HWGBS2072 | 3.35 | 7.09 | −3.78 | |
| Qfla.hww-5A | 5A | 28 | HWGBS3329-HWGBS3337 | 7.98 | 15.02 | −4.24 | ||
| M16 | Qfla.hww-2D.c | 2D | 59 | HWGBS1931-HWGBS1938 | 3.49 | 9.60 | 3.78 |
1Environment. 2Chromosome where the QTL was located. 3Chromosome position (cM) of the QTL. 4Phenotypic variance explained. 5Additive effect (positive values of the additive effect indicated that alleles from parent ‘Harry’ were in the direction of increasing trait score and negative indicated that alleles from parent ‘Wesley’ were in the direction of increasing trait score).