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. 2017 Nov 27;7:16385. doi: 10.1038/s41598-017-16685-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification and characterization of distinct cell subpopulations within VMP and SU tissue compartments. (a) tSNE analysis identified markers of cell subpopulations that separates both VMP and SU in to two distinct VMP and two distinct SU cell subpopulations labeled clusters 1 through 4 respectively. (b) Expression heatmap of most discriminatory cluster markers (avg_diff > 0 and AUC > 0.75) across VMP and SU cell populations. Expression values presented as log₂ (TPM + 1) (TPM). (c) Violin plots showing expression of selected cluster marker genes across VMP and SU cell populations. Expression is presented as log₂ (TPM + 1). Width of the violin plot indicates frequency of cells with that expression level.