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. 2017 Nov 27;7:16345. doi: 10.1038/s41598-017-16558-0

Figure 1.

Figure 1

The EPE peptide reduces the viability of some BRAF, NRAS and NF1 mutant melanoma cell lines. (A) Effect of the EPE peptide on proliferation of metastatic melanoma cells. Thirty-eight melanoma cell lines derived from metastatic tumor resections were treated with either EPE or scrambled (Scr) peptides (10 µM). A subset of BRAF, NRAS and NF1 mutant melanomas were sensitive to the EPE peptide. Bars in white correspond to cell lines selected for further study. (B) Effect of BRAF inhibitor vemurafenib on selected melanomas. Four EPE-sensitive NRAS melanomas (63T, 83T, 120T and 60T), and three EPE-resistant melanomas (110T, 39T, 103T) were treated either with vemurafnib (1 µM, Vem), EPE or Scr peptides (10 µM). All melanoma cells selected were resistant to BRAF inhibition. Cell viability was measured by CellTiter-Glo reagent after 96 h of treatment. Bars represent percentage of growth respect to Scr peptide control ± S.E. of 3 independent experiments.