Fig. 1.

NKX2.1-GFP hESC reporter line can be directed toward an OPC fate.

(a) Directed differentiation of hESC into OPCs. (b) hESCs (stage I) were differentiated to neural embryoid bodies (EBs) (stage II) expressing (c, d) NKX2.1. (e) Nestin + neural precursor cells (stage III) were generated. (f) Olig2 + Glial precursor cells (stage IV) appeared under the influence of EGF. These were further differentiated into OPCs (stage V) expressing (g) PDGFRα and (h) NG2 through the addition of PDGF-AA. (i, j) T₃ promoted terminal differentiation of OPCs into (i) O4 + pre-OLs (stage VI), and (j) MBP + pre-myelinating OLs. (e–j) Counterstaining was performed by DAPI. Scale bar = 100 μm for (b–d) and 20 μm for (e–j).