Fig. 4.

Transcriptome analysis for human mixed neural cell cultures differentiated toward OPC lineage with DITPA.

(a) The proportion of NG2-positive cells during differentiation of Merck-Millipore human mixed neural cell cultures. One-way ANOVA with Tukey's post-hoc test; ****P < 0.0001 (n = 3–4; mean ± SEM). At week 4, ~ 30% of NG2 + cells were derived and DITPA (10 ng/mL) was treated (circled in red). (b) DITPA treatment (10 ng/mL) regime upon human OPC specification from neural precursors. (c) Comparative gene expression profiles from different concentrations of DITPA (10 ng/mL) versus ethanol control (including T₃ in differentiation medium) treatments. (d) Genome-wide transcriptional profile heatmap obtained for the different concentrations of DITPA (10 ng/mL) and ethanol control treatments. (e) Highly enriched GO terms of commonly expressed genes following the administration of DITPA at 1 ng/mL and 10 ng/mL versus ethanol control. (f and g) Heatmap of (f) genes related to TH signaling and (g) Neuronal, astroglial and oligodendroglial enriched transcription factors (Gene lists are from Najm et al., 2013) following the administration DITPA (10 ng/mL) and ethanol control.