Fig. 6.

DITPA promotes the myelination of rat RGCs from NKX2.1-GFP + hESC derived OPCs.

(a–d) At day 10, stage VI, OPCs from NKX2.1-GFP + sorted cells were seeded on rat RGCs and maintained for 7 days with T₃ (50 ng/mL), DITPA (10 ng/mL), or T₃ (50 ng/mL) + DITPA (10 ng/mL) treatment, then immunostained with MBP, NF-200, and DAPI. MBP + OLs were scored for their morphology as (a) “Resting”, “Contacting”, or “Ensheathing”. (b) Representative deconvoluted z-stack captured images from the myelinating co-cultures treated with T₃ (50 ng/mL), DITPA (10 ng/mL) or T₃ (50 ng/mL) + DITPA (10 ng/mL). For better representation, these z-stack images were rendered into an artificial 3D image and shown below as raw images (arrowhead indicates regions of myelination, scale bars = 50 μm). (c) The percentage of MBP + OLs that are resting, contacting, and ensheathing from the different treatment groups. (d) The percentage of myelinated axons within the different treatment groups. One-way ANOVA with Tukey's post-hoc analysis; *P < 0.05; ***P < 0.001; ****P < 0.0001; (n = 9–10, mean ± SEM).