Table 1.
Contraction and relaxation of blood vessels from 19‐day chicken embryos exposed to hypoxia‐reoxygenation
| Blood vessel | Experimental group | Contraction | Relaxation | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KCl (62.5 mmol/L) | NE (10 μmol/L) | ACh (10 nmol/L‐0.1 mmol/L) | SNP (10 nmol/L–0.1 mmol/L) | Forskolin (10 nmol/L–10 μmol/L) | ||||||||||
| mN/mmol/L | n | mN/mmol/L | n | E max | pEC50 | n | E max | pEC50 | n | E max | pEC50 | n | ||
| Pulmonary artery | NormOx‐NormOx | 0.18 ± 0.03 | 18 | – | – | 74.9 ± 5.9 | 7.07 ± 0.1 | 6 | 97.2 ± 6.1 | 6.31 ± 0.1 | 6 | 102.2 ± 8.3 | 5.82 ± 0.2 | 6 |
| HypOx‐NormOx | 0.15 ± 0.03 | 18 | – | – | 71.3 ± 4.9 | 6.88 ± 0.2 | 6 | 94.3 ± 7.9 | 6.46 ± 0.2 | 6 | 104.3 ± 9.1 | 5.64 ± 0.2 | 6 | |
| HypOx‐HyperOx | 0.17 ± 0.03 | 17 | ‐ | ‐ | 77.9 ± 6.2 | 6.96 ± 0.4 | 6 | 96.4 ± 6.3 | 6.36 ± 0.3 | 6 | 97.4 ± 8.3 | 5.71 ± 0.3 | 6 | |
| NormOx‐HyperOx | 0.14 ± 0.04 | 17 | – | – | 70.2 ± 6.4 | 6.81 ± 0.2 | 6 | 101.2 ± 8.1 | 6.51 ± 0.2 | 6 | 96.2 ± 8.7 | 5.63 ± 0.2 | 6 | |
| Mesenteric artery | NormOx‐NormOx | 0.94 ± 0.18 | 18 | 0.88 ± 0.11 | 18 | 103.2 ± 8.1 | 6.06 ± 0.2 | 7 | 101.4 ± 8.7 | 6.32 ± 0.1 | 7 | 97.2 ± 8.7 | 6.86 ± 0.3 | 9 |
| HypOx‐NormOx | 0.78 ± 0.17 | 19 | 0.83 ± 0.14 | 19 | 96.3 ± 8.2 | 5.98 ± 0.2 | 7 | 96.3 ± 8.8 | 6.24 ± 0.1 | 7 | 106.3 ± 9.5 | 7.01 ± 0.3 | 8 | |
| HypOx‐HyperOx | 0.89 ± 0.11 | 17 | 0.86 ± 0.14 | 17 | 106.4 ± 8.3 | 6.14 ± 0.3 | 7 | 107.0 ± 8.6 | 6.15 ± 0.2 | 6 | 103.4 ± 8.8 | 6.94 ± 0.3 | 7 | |
| NormOx‐HyperOx | 0.88 ± 0.14 | 17 | 0.76 ± 0.18 | 17 | 102.2 ± 9.1 | 5.89 ± 0.3 | 8 | 106.0 ± 10.2 | 6.25 ± 0.3 | 7 | 95.2 ± 7.8 | 6.87 ± 0.3 | 7 | |
| Femoral artery | NormOx‐NormOx | 1.63 ± 0.18 | 19 | 2.21 ± 0.19 | 19 | 98.2 ± 10.4 | 6.96 ± 0.3 | 6 | 101.3 ± 8.6 | 5.91 ± 0.2 | 8 | 98.6 ± 9.7 | 5.96 ± 0.3 | 6 |
| HypOx‐NormOx | 1.71 ± 0.21 | 19 | 1.97 ± 0.22 | 19 | 107.3 ± 9.2 | 6.98 ± 0.3 | 7 | 104.2 ± 9.1 | 5.81 ± 0.3 | 7 | 96.3 ± 9.1 | 5.94 ± 0.2 | 7 | |
| HypOx‐HyperOx | 1.83 ± 0.13 | 17 | 2.31 ± 0.26 | 17 | 101.9 ± 10.3 | 6.88 ± 0.3 | 7 | 98.2 ± 10.1 | 5.78 ± 0.2 | 8 | 101.4 ± 7.8 | 6.01 ± 0.3 | 8 | |
| NormOx‐HyperOx | 1.69 ± 0.16 | 17 | 2.11 ± 0.20 | 17 | 96.2 ± 8.1 | 7.01 ± 0.3 | 6 | 97.3 ± 9.2 | 5.93 ± 0.3 | 7 | 105.2 ± 10.8 | 6.03 ± 0.3 | 7 | |
| Ductus arteriosus | NormOx‐NormOx | 0.38 ± 0.05 | 19 | 0.45 ± 0.08 | 19 | 93.2 ± 7.4 | 6.81 ± 0.3 | 7 | 98.3 ± 6.4 | 6.52 ± 0.3 | 9 | 97.2 ± 8.7 | 6.32 ± 0.2 | 8 |
| HypOx‐NormOx | 0.48 ± 0.06 | 19 | 0.44 ± 0.06 | 19 | 96.4 ± 10.1 | 6.91 ± 0.3 | 7 | 104.3 ± 8.9 | 6.54 ± 0.2 | 8 | 106.3 ± 9.5 | 6.24 ± 0.3 | 7 | |
| HypOx‐HyperOx | 0.51 ± 0.06 | 17 | 0.52 ± 0.06 | 17 | 90.4 ± 7.3 | 6.72 ± 0.3 | 6 | 96.7 ± 8.6 | 6.45 ± 0.3 | 7 | 103.4 ± 8.8 | 6.33 ± 0.1 | 8 | |
| NormOx‐HyperOx | 0.44 ± 0.05 | 17 | 0.47 ± 0.05 | 17 | 92.2 ± 8.7 | 6.78 ± 0.3 | 6 | 102.2 ± 9.1 | 6.37 ± 0.4 | 8 | 95.2 ± 7.8 | 6.39 ± 0.1 | 7 | |
Eggs were exposed to 10% O2 for 30 min followed by 15 min of reoxygenation with 21% O2 (HypOx‐NormOx) or 80% O2 (HypOx‐HyperOx). A group of eggs was maintained at 21% O2 for 45 min (NormOx‐NormOx) and a group of eggs was maintained at 21% 02 for 30 min and then exposed to 80% O2 for 15 min (NormOx‐HyperOx). Results are shown as mean ± SE of measurements in n embryos. ACh, acetylcholine; NE, norepinephrine; SNP, sodium nitroprusside.