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. 2017 Oct 26;7:76–85. doi: 10.1016/j.omto.2017.10.003

Figure 4.

Figure 4

Tumor Cell Lysis Activity of the TRAD Expressing a Dominant-Negative IκBα in Human Non-hepatic Tumor Cells

(A) A549 and PH5CH8 cells were infected with TRAD-DNIκBα at an MOI of 5. After 48-hr incubation, the DNIκBα mRNA levels were determined by qRT-PCR analysis. ***p < 0.001. (B) A549 cells were transfected with pNF-κB-Luc, followed by infection with the conventional TRAD or TRAD-DNIκBα at an MOI of 5. After 48-hr incubation, luciferase activity was determined. The data show FLuc activity normalized by RLuc activity. (C–E) A549 cells were infected with the conventional TRAD or TRAD-DNIκBα at an MOI of 5. After 48-hr incubation, the E1A, E2, and E4 mRNA levels (C), the copy numbers of TRAD genomic DNA (D), and the IFU titers of progeny TRAD (E) in the cells were determined by qRT-PCR analysis, qPCR analysis, and infectious titer assay, respectively. (F) A549, H1299, PancI, and MDA-MB-231 cells were infected with the conventional TRAD or TRAD-DNIκBα at the indicated MOIs. After 5-day incubation, cell viability was determined by alamarBlue assay. The data were normalized by the data of the mock-infected group. Data are the means ± SD (n = 4).