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. 2017 Oct 30;9(11):149. doi: 10.3390/cancers9110149

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of the importance of Y1283 for ALK-Y1278S activity. (A) Representative images of PC12 cells expressing pcDNA3 empty vector, wild type ALK, ALK-Y1278S, Y1283F or ALK-Y1278S mutants respectively. Scale bar represents 50 μm; (B) Detection of phosphorylation of ALK (Y1604) and downstream signalling activity (pERK1/2) of wild type ALK, ALK-Y1278S, Y1283F and ALK-Y1278S in the absence or presence of ALKAL1 ligand by immunoblotting. Blots are representative of three independent experiments; (C) Neurite outgrowth assay of PC12 cells transfected with various ALK mutants as indicated in the presence or absence of ALKAL1 stimulation. Bars represent mean percentage ± STD of neurite-carrying cells among GFP-positive cells from three independent experiments.