Table 3.
Diagnostic performance of cell line secretome markers validated in blood.
| First Author, Year, [Ref] | Proteins | Sensitivity (95%CI) % | Specificity (95%CI) % | Area under the Curve (95%CI) | Method | Validation | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CEA | CA19-9 | ACVR2B | AZGP1 | GDF15 | LRG1 | S100A9 | MAPKAP3 | TIMP1 | PIM1 | Others | ||||||
| Cell Line Secretome Combination Marker Studies | ||||||||||||||||
| Wu, 2008 [35] | x | CRMP2 | 77 (71–83) | 95 (91–98) | 0.75 (0.68–0.78) | ELISA | No | |||||||||
| Wu, 2008 [36] | x | Mac-2BP | 69 (63–74) | 72 (64–79) | 0.774 (0.727–0.816) | ELISA | No | |||||||||
| Babel, 2009 [50] | x | x | x | 84 (71–93) | 71 (55–84) | 0.85 | ELISA | BS | ||||||||
| Tagi, 2010 [40] | x | DK, S-p53 (Stg.1) | 58 (48–68) | 80 (71–87) | - | ELISA | No | |||||||||
| Babel, 2011 [51] | SULF1, MST1/STK4 & phages NHSL1, GTF2i, SREBF2, GRN | 83 (70–92) | 70 (55–83) | 0.86 | ELISA | BS | ||||||||||
| Fan, 2011 [38] | x | SEC61β | 71 (60–80) | 89 (79–95) | 0.838 (0.774–0.903) | ELISA, WB | No | |||||||||
| Weiss, 2011 [59] | x | sE-cadherin | 34 (22–48) | 97 (87–100) | - | ELISA | No | |||||||||
| Barderas, 2012 [52] | x | x | x | FGFR4 & phages GRN, NHSL1, SREBF2 | 89 (77–96) | 90 (78–97) | 0.925 | ELISA | BS | |||||||
| Ladd, 2012 [65] | x | x | IGFBP2, MAPRE1 | 41 (24–60) | 95 (81–100) | 0.724 | ELISA | SS | ||||||||
| Lee, 2012 [48] | x | HMGB1 | 42 (35–49) | 87 (77–94) | 0.643 | ELISA | No | |||||||||
| Barderas, 2013 [53] | x | x | S100A8/A9, SERPINI1 | 60 (43–75) | 95 (75–100) | 0.884 | ELISA | No | ||||||||
| Shin, 2014 [46] | x | PAI-1, TRFM | 68 (62–74) | 90 (81–96) | 0.821 (0.731–0.890) | ELISA | No | |||||||||
| Chiang, 2015 [37] | x | BST2 | 24 (17–33) | 100 (97–100) | 0.872 (0.828–0.916) | ELISA | SS | |||||||||
| Lin, 2015 [49] | x | LAMB1 | 80 (65–90) | 92 (80–98) | 0.911 (0.85–0.97) | ELISA | No | |||||||||
| Taguchi, 2015 [67] | x | MAPRE, AK1 | 43 (30–56) | 95 (86–99) | - | ELISA | BS | |||||||||
| Cell Line Secretome Individual Marker Studies | ||||||||||||||||
| Nam, 2005 [66] | Defensin α6 | 69 (54–81) | 83 (58–96) | - | ELISA | SS | ||||||||||
| Xue, 2010 [28] |
x |
TFF3 | 54 (46–62) 78 (70–85) |
97 (93–99) 99 (96–100) |
0.730 (0.670–0.791) 0.897 (0.856–0.938) |
ELISA | No | |||||||||
| Rodriguez-P, 2012 [54] | Clusterin | 81 (63–93) | 79 (61–91) | 0.845 (0.747–0.942) | ELISA | No | ||||||||||
| Toiyama, 2014 [43] | ANGPTL2 | 70 (63–76) | 96 (86–100) | 0.885 (0.838–0.923) | ELISA | SS | ||||||||||
| Qiao, 2015 [24] | COL6A3 | 93 (81–99) | 81 (67–91) | 0.885 | ELISA | No | ||||||||||
| Zhang, 2015 [33] | Spondin-2 | 100 (92–100) | 90 (76–97) | 0.959 | ELISA | No | ||||||||||
| Fan, 2016 [31] |
x |
x |
MRC1 | 74 (65–82) 81 (73–88) 52 (42–62) |
63 (53–73) 80 (71–88) 48 (38–58) |
0.744 (0.678–0.810) 0.873 (0.826–0.920) 0.464 (0.384–0.544) |
ELISA | No | ||||||||
| Wang X, 2016 [26] |
x |
COL3A1 | 99 (94–100) 70 (59–79) |
69 (57–80) 73 (61–83) |
0.92 0.791 |
ELISA | No | |||||||||
Abbreviations: BS: Bootstrap method; ELISA: Enzyme-linked immunosorbent assay; SS: Split Sampling method; WB: Western Blot. Abbreviations for proteins: ACVR2B: Activin receptor type-2B; AK1: Adenylate Kinase 1; ANGPTL2: Angiopoietin-like protein 2; AZGP1: Zinc-α-2-glycoprotein; BST2: Bone marrow stromal antigen 2; CA 19-9: Carbohydrate antigen 19-9; CEA: Carcinoembryonic antigen; COL3A1: Collagen alpha-1(III); COL6A3: Collagen alpha-3(VI) chain; CRMP-2: Collapsin response mediator protein-2; DK: Dermokine; GDF15: Growth/differentiation factor 15; GRN: Granulin; GTF2i: General transcription factor; FGFR4: Fibroblast growth factor receptor 4; HMGB1: High mobility group protein B1; IGFBP2: Insulin like growth factor binding protein 2; LAMB1: Laminin β-1; LRG1: Leucine-rich alpha-2-glycoprotein; MAC-2BP/TAA90K: Mac-2 binding protein/Tumor-associated antigen 90 K; MAPKAPK3: Mitogen-activated protein kinase-activated protein kinase 3; MAPRE1: Microtubule-associated protein RP/EB family member 1; MRC1: Macrophage mannose receptor 1; MST1/STK4: Mammalian STE20-like protein kinase 1; NHSL1: NHS-like protein 1; PAI-1: Plasminogen activator inhibitor-1; PIM1: Serine/threonine-protein kinase pim-1; S-p53: Serum-p53; S100A8/A9: S100 calcium-binding protein A8/A9; SEC61β: Protein transport protein Sec61 subunit beta; SERPINI1: Neuroserpin; SREBF2: Sterol regulatory element binding protein 2; SULF1: Sulfatase 1; TFF3: trefoil factor; TIMP1: Tissue inhibitor of metalloproteinases 1; TRFM: Melanotransferrin; -: means not specified by the study; x: this type of protein was identified by the study.