Fig. 2.

Membrane association of Grx1-roGFP2^Palm and roGFP2-Orp1^Palm assessed by fluorescence recovery after photobleach (FRAP) experiments. A, Confocal images of HeLa cells expressing fluorescent redox sensors. Bleaching laser was shot on 10 µm × 10 µm area (dashed square) and images were taken after indicated time periods. As non-diffusible controls, cells were fixed with 3.7% paraformaldehyde prior to FRAP (Grx1-roGFP2 Fixed). B, FRAP kinetics for cells shown in A.