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. 2017 Nov 21;14:679–685. doi: 10.1016/j.redox.2017.11.015

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 3 — Palmitoylation did not affect reactivity of Grx1-roGFP2 and roGFP2-Orp1. Grx1-roGFP2^Palm and roGFP2-Orp1^Palm were transfected into HeLa cells. Redox reactivity was tested by sequential treatment with 100 µM H₂O₂ (oxidant, Time = 10 min) and 5 mM DTT (reductant, Time = 40 min in A and 35 min in B) during live cell imaging. I405 and I488 Images were taken by confocal microscopy and the ratio R_I405/I488 was obtained for each cell. Time-dependent changes of R_I405/I488 values for Grx1-roGFP2^Palm (A) and roGFP2-Orp1^Palm (B) are shown. PM, plasma membrane. VM, vesicular membrane.