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Peripheral blood neutrophils-induced FLCN expression and release from A549 cells. A549 cells (2 × 105 cells) were co-cultured with peripheral blood neutrophils for 24 h. (a) Intracellular FLCN expression was evaluated by western blotting. (b) The levels of FLCN released to the culture supernatants were measured by ELISA. *P<0.05, **P<0.01, ***P<0.001 vs control, obtained by one-way ANOVA with Bonferroni’s post hoc test. FLCN, folliculin.
