Figure 3.

CORM-2 inhibits TNF-α-mediated biogenesis of miR-155-5p that targets the 3′-UTR of eNOS. HUVECs were transfected with or without MIR155HG promoter-Luc construct or psiCHECK-2-eNOS 3′-UTR reporter constructs (wild type, WT; mutant, MT) and stimulated with TNF-α in the presence of RuCl₃ (200 μM) or CORM-2 (200 μM). (a) MIR155HG expression was determined by RT-PCR (upper) and qRT-PCR (lower). (b) MIR155HG promoter-Luc activity was determined in cell lysates. (c) Precursor and mature miR-155-5p levels were determined by qRT-PCR. (d) Luciferase activity of eNOS 3′-UTR was determined in cell lysates. n=3. **P<0.01; ***P<0.001.