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FISH using Mli2 (Fluorescein, green) and Mli1dist (TAMRA, tetramethylrhodamine, red) on abnormal metaphase plates of M. lignano. (A) Three metaphase plates with the abnormal 2n = 8 chromosome set (two large metacentrics, one unpaired medium-sized acrocentric, and five small metacentrics); (B) abnormal 2n = 10 chromosome set (four large metacentrics, four small metacentrics, and two small acrocentrics). Rearranged chromosomes are marked with arrows. Scale bar: 10 µm.
