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Primers used for the amplification of unique DNA sequences.
| Unique DNA Fragment | Primer Sequence | PCR Product Length | |
|---|---|---|---|
| R1 | F | CGGTACTCCTTCCAGGACATTG | 20,124 bp |
| R | CGCAACAGTGACGCTAACTATC | ||
| R2 | F | CACGTAGACGGTCATACGAGTT | 20,232 bp |
| R | CCGCTAATCCACGTCATGACTA | ||
| R3 | F | CTGACTGGGTGCGAATCCAT | 20,254 bp |
| R | AAACCTATGTCCTAGCTGTGCG | ||
| R4 | F | GTCCTGGAACTGTCTGTACACC | 20,200 bp |
| R | GTTTCGGGCAAACGATAGCTAC | ||
| R5 | F | AAAAATCTGCCGCCATTTGTGA | 20,278 bp |
| R | ATGAATGTTGACTCGGAAGGCT |
All primers are 5′-3′. F: forward; R: reverse.
