Table 2.
Comparative content of NADHox mRNA in the transformants synthesizing dsRNA from 200 to 500 bp with respect to the WT strain, as measured by RT-qPCR.
| Transformants | Mean mRNA Content (±SD) 2 | Percent mRNA Content Relative to WT (%) |
|---|---|---|
| WT 1 | 1 (±0.041) | 100 |
| N200 | 0.697 (±0.015) | 69.7 |
| N300 | 0.885 (±0.079) | 88.5 |
| N400 | 0.837(±0.021) | 83.5 |
| N500 | 1.028 (±0.089) | 100 |
1 Wild type (WT) is considered to have a mRNA content value of 1.0, and the mRNA content of the transformants is relative to this. 2 Values are the result of three replicate experiments, using Act as housekeeping reference gene for normalization.