Figure 3.

LincRNA association with protein coding genes and clinical variables. (A) Fold change (FC) comparison of differentially expressed lincRNAs and their adjacent protein coding genes. Most of them are in coordinated changes, i.e., both either up or down expressed as the dots shown in quadrant I and III; (B) Unsupervised clustering with clinical variables. Stage: dark blue for stage IA and light green for stage IB; Cell: A—adenocarcinoma, B—adenocarcinoma with bronchoalveolar carcinoma feature (or lepidic predominant adenocarcinoma in the new classification); Grade: W—well differentiated and M—intermediately differentiated; Status: patient survival status, 1—dead and 0—alive; EGFRmut: EGFR mutation status, N—none, L—L858R substitution, I—insertion/deletion, A750P substitution; (C) Kaplan-Meier survival curves by clusters defined by lincRNA expression; (D) lincRNA differentiation and association with patient survival. lincRNAs that were highly expressed in tumors also led to worse survival (positive hazard ratio). Conversely those expressed lower had reduced risk to patient survival.