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. 2017 Sep 21;14(5):4671–4680. doi: 10.3892/etm.2017.5160

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of ERK1/2-siRNA on the expression of ERK1/2 and cyclin E1 in the pulmonary vessels of cigarette smoke-exposed rats. (A) The mRNA levels of ERK1/2 and cyclin E1. (B) Protein levels of ERK1/2 and cyclin E1 were evaluated by western blot analysis. Immunostaining for (C) p-ERK1/2 and (D) cyclin E1 was also performed in each group (C) p-ERK or (D) cyclin E1. Blue, indicated the nucleus; red, indicated the cytoplasm; and dark brown indicated p-ERK or cyclin E1. Magnification, ×200. *P<0.05 vs. control. ERK, extracellular signal-regulated kinase; siRNA, small interfering RNA; NC, negative control.