Figure 3.

The relationship between genetic variation of rs3811463 and SIRT1 protein level. Muscle cells were pretreated in low-glucose DMEM for 12 h before 4 h of 10 µg/ml of insulin treatment, and then transfected with pEF6/V5-His-TOPO (Ctrl group), pEF6/V5-His-TOPO: rs3811463T (rs3811463T group) or pEF6/V5-His-TOPO: rs3811463C (rs3811463C group), respectively. Cells were collected and protein was extracted with RIPA buffer for western blot. All experiments were carried out in triplicate independent repeat. All data were analyzed by one-way ANOVA. Compared with Ctrl group, ^aP<0.05; Compared with rs3811463T group, ^cP<0.05.