Figure 1.

Successful transduction of adenoviral vectors into pancreatic β-cells. The pancreatic islets were infected with adenoviruses containing the pHBAd-MCMV vector, pHBAd-MCMV-TRPM2, pHBAd-U6-control shRNA (scramble shRNA) or pHBAd-U6-TRPM2 shRNA (shTRPM2). After 24 h, the expression levels of TRPM2 were examined by (A) reverse transcription-quantitative polymerase chain reaction analysis and (B) western blot analysis. n=3. *P<0.05 vs. the control group. TRPM2, transient receptor potential melastatin 2; O/E, overexpression; shRNA, short hairpin RNA; shTRPM2, shRNA directed against TRPM2.