Figure 2.

Glucose-stimulated insulin secretion is attenuated by TRPM2 knockdown in primary pancreatic islets. (A) A whole-cell patch clamp experiment was performed on pancreatic β-cells in the presence of 100 µM ADP-ribose, revealing that the current flow was eliminated in TRPM2-silenced cells. The plot is the representative current flow chart. (B) The peak current was recorded at three glucose concentrations. Only a high glucose concentration (16.6 mM) potentiated the current in the negative control islets, while TRPM2 silencing inhibited this effect. Measurements were taken in the (C) first (0–10 min) and (D) second (10–60 min) phases of insulin secretion. n=8. *P<0.05. shRNA, short-hairpin RNA; TRPM2, transient receptor potential melastatin 2; shTRPM2, shRNA directed against TRPM2.