Figure 3.
GLP-1 potentiates glucose-stimulated insulin secretion via TRPM2 rather than through K+-induced insulin release. (A) Whole-cell currents were recorded in response to 5.6 mM glucose. When administered, 10 nM GLP-1 increased TRPM2 current with or without the addition of 30 mM KCl. (B) Insulin release was measured at a low (3.3 mM) and high (16.6 mM) glucose concentration. n=9. *P<0.05. #, &, §, ΔP<0.05 between the two groups with the same label. shRNA, short-hairpin RNA; TRPM2, transient receptor potential melastatin 2; shTRPM2, shRNA directed against TRPM2; ns, not significant; GLP-1, glucagon-like protein-1.