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. 2017 Sep 22;14(5):4910–4918. doi: 10.3892/etm.2017.5196

Figure 2.

Figure 2.

Western blot analysis of TLR4 expression and NF-κB p65 activity in spinal dorsal horn of Sham, CFA, DEX, EGCG and B groups. (A and B) Effects of intrathecal injection with DEX, BRL44408 and EGCG for 3 consecutive days on the (A) NF-κB p65 and (B) TLR4 levels in the spinal dorsal horn induced by MA on days 3 and 7 after MA, respectively. GAPDH served as loading control. **P<0.05, SHAM vs. CFA; #P<0.01 and ##P<0.05, DEX vs. CFA; £P<0.01 and ££P<0.05, DEX vs. B; &P<0.01 and &&P<0.05, EGCG vs. CFA; $P<0.01 and $$P<0.05, EGCG vs. B. Sham, i.a. injection with 50 µl sterile saline and i.t. injection with 20 µl sterile saline for 3 consecutive days; CFA, i.a. injection with 50 µl CFA; DEX, i.a. injection with 50 µl CFA and i.t. injection with DEX (2.5 µg/20 µl) for 3 consecutive days; B, i.a. injection with 50 µl CFA and pre-treatment with BRL44408 (15 µg/20 µl) 30 min prior to i.t. injection with DEX 2.5 (µg/20 µl) for 3 consecutive days; EGCG, i.a. injection with 50 µl CFA and i.t. injection with EGCG 30 µg for 3 consecutive days. i.t., intrathecal; i.a., intra-articular; TLR, Toll-like receptor; NF, nuclear factor; CFA, complete Freund's adjuvant; MA, monoarthritis; DEX, dexmedetomidine; EGCG, epigallocatechin gallate; B, BRL44408.