Figure 5.

(A) Double immunofluorescence revealed that GFAP was co-localized with α2AR and TLR4 immunoreactivity in the spinal dorsal horn of the CFA group at 3 days. Arrowheads indicate double-labeled cells. (B) Co-immunoprecipitation assay. α2AR was detected by with specific antibodies to TLR4, and TLR4 was also detected with specific antibodies to α2AR, as was the positive control. However, when IgG was used as a negative control, no immunoreactivity was detected. Magnification, ×20. IgG, immunoglobulin G; GFAP, glial fibrillary acid protein; TLR, Toll-like receptor; α2AR, α 2-adrenoceptors.