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. 2017 Nov 8;3(11):1156–1167. doi: 10.1021/acscentsci.7b00338

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Copyright © 2017 American Chemical Society

This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

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Dnm1 generates NGC necessary for mitochondrial membrane fission. (A) SAXS spectra from 75/15/10 and 75/5/20 PE/PC/CL model mitochondrial membranes incubated with Dnm1 at P/L = 1/1000. Correlation peaks corresponding to assigned reflections are indicated for hexagonal (green) and cubic (black lines) phases. (B) 3D reconstructions depicting Im3m (left) and Pn3m (right) cubic phases, which have continuous surfaces with NGC at every point. (C) Indexing of Dnm1-induced H_II, and Pn3m and Im3m Q_II phases for 75/15/10 (red) and 75/5/20 (blue) PE/PC/CL membranes. Plots of the measured Q positions, Q_measured, versus the assigned reflections in terms of Miller indices, for hexagonal and for cubic phases. The lattice parameters were calculated from the slopes of the linear regressions through the points and are provided in the legend.

Inline graphic — Dnm1 generates NGC necessary for mitochondrial membrane fission. (A) SAXS spectra from 75/15/10 and 75/5/20 PE/PC/CL model mitochondrial membranes incubated with Dnm1 at P/L = 1/1000. Correlation peaks corresponding to assigned reflections are indicated for hexagonal (green) and cubic (black lines) phases. (B) 3D reconstructions depicting Im3m (left) and Pn3m (right) cubic phases, which have continuous surfaces with NGC at every point. (C) Indexing of Dnm1-induced H_II, and Pn3m and Im3m Q_II phases for 75/15/10 (red) and 75/5/20 (blue) PE/PC/CL membranes. Plots of the measured Q positions, Q_measured, versus the assigned reflections in terms of Miller indices, for hexagonal and for cubic phases. The lattice parameters were calculated from the slopes of the linear regressions through the points and are provided in the legend.