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. 2017 Nov 8;3(11):1156–1167. doi: 10.1021/acscentsci.7b00338

Figure 5.

Figure 5

Dnm1 exhibits reduced ability to generate NGC in the presence of Fis1. (A) Dnm1 (P/L = 1/1000) in combination with Fis1 (P/L = 1/500) restructured both 75/15/10 and 75/5/20 PE/PC/CL model membranes but with decreased membrane curvature induction compared to Dnm1 alone (Figure 2). Correlation peaks corresponding to assigned reflections are indicated for hexagonal (green) and cubic (black lines) phases. (B) Indexing of generated HII, and Im3m and Pn3m QII phases for 75/15/10 (red) and 75/5/20 (blue) PE/PC/CL membranes reveals increases in the lattice parameters for all induced phases compared to membranes incubated only with Dnm1 (Figure 2C). These larger lattice parameters and no observed Pn3m QII phase for 75/15/10 PE/PC/CL indicate a marked attenuation in overall curvature induction and a reduced ability of Dnm1 to generate NGC.