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. 2017 Sep 21;14(5):4789–4796. doi: 10.3892/etm.2017.5180

Figure 2.

Figure 2.

Effect of ethanol treatment on the expression of claudin-5, occludin and ZO-1. Protein expression levels were measured using western blotting, and mRNA expression levels were determined using reverse transcription-quantitative polymerase chain reaction. (A) Western blotting analysis of claudin-5, occludin, ZO-1 and β-actin expression following ethanol treatment at (A) 24, (B) 48 and (C) 72 h. Quantitative analysis of western blotting results, which were normalized to β-actin expression at (D) 24, (E) 48 and (F) 72 h. Relative expression of claudin-5, occludin and ZO-1 mRNA at (G) 24, (H) 48 and (I) 72 h. The results (n=5) are presented as the mean ± standard deviation. *P<0.05 vs. control at 24 h; #P<0.05 vs. control at 48 h; ^P<0.05 vs. control at 72 h. ZO-1, zonula occludens-1.