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. 2017 Sep 21;14(5):4789–4796. doi: 10.3892/etm.2017.5180

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of the ethanol-induced increase in p-PKCα expression on claudin-5, occludin and ZO-1 expression. (A) Western blotting analysis of p-PKCα and β-actin protein expression. (B) Quantitative analysis of p-PKCα protein expression relative to β-actin expression. (C) Western blotting analysis of claudin-5, occludin and ZO-1 protein expression. (D) Quantitative analysis of claudin-5, occludin and ZO-1 protein expression relative to β-actin expression. The results (n=5) are presented as the mean ± standard deviation. *P<0.05 vs. control; ^#P<0.05 vs. 200 mM ethanol. p-PKCα, phosphorylated-protein kinase Cα; ZO-1, zonula occludens-1; S, safingol.