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Primer sequences used for reverse transcription-quantitative polymerase chain reaction.
| Genes | Forward (5′-3′) | Reverse (5′-3′) | GenBank ID |
|---|---|---|---|
| Claudin-5 | TTTCCCTAACTTCAGCTGCC | CCCTCTTTGAAGGTTCGGG | NM_001130861 |
| Occludin | GCAAAGTGAATGACAAGCGG | CACAGGCGAAGTTAATGGAAG | NM_002538 |
| ZO-1 | TGCTGAGTCCTTTGGTGATG | AATTTGGATCTCCGGGAAGAC | NM_003257 |
| β-actin | CTAACTTGCGCAGAAAACAAGAT | TTCCTGTAACAACGCATCTCATA | NM_001101 |
ZO-1, zonula occludens-1.
