Figure 2.

Antioxidant activity of BAW. (A) DPPH radical scavenging activity was assayed in a mixture containing different concentrations of BAW. IC₅₀ reflects the substrate concentration required to produce a 50% reduction in DPPH activity. (B) Determination of intracellular ROS production. After DCFH-DA treatment, green fluorescence in subset groups of cells was observed at 200× magnification using a fluorescence microscope (Eclipse TX100, Nikon, Tokyo, Japan). Arrows indicate cells stained with DCFH-DA.