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. 2017 Sep 25;14(5):4986–4994. doi: 10.3892/etm.2017.5200

Figure 5.

Figure 5.

Measurement of pro-inflammatory and anti-inflammatory cytokines. The mRNA levels of the TNF-α (A and Ba), IL-1β (A and Bb), and IL-6 (A and Bc) genes were examined by RT-PCR in the No-, Vehicle+LPS-, BAWLo+LPS-, and BAWHi+LPS-treated group using specific primers. (Bd) IL-6 concentration in the culture supernatant. After collection of culture supernatant from RAW264.7 cells co-treated with BAW+LPS, IL-6 concentrations were measured by using a IL-6 ELISA kit that could detect IL-6 at 9.3 pg/ml. The data shown represent the means ± SD of three replicates. *P<0.05 relative to the No-treated group. #P<0.05 relative to the Vehicle+LPS-treated group.