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. 2017 Sep 21;14(5):4781–4788. doi: 10.3892/etm.2017.5178

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — LAMP is more sensitive in the detection of cdtB than PCR amplification. Comparative sensitivities of cdtB detection by (A) real-time turbidimeter observations of the LAMP assay, (B) visual observation of the LAMP assay and (C) traditional PCR. Tubes 1–9 and lanes 1–9 contain a 10-fold serial dilution of pure genomic DNA extracted from C. difficile (from 24.8 ng/µl to 0.000248 pg/µl); tube 10 and lane 10 contain ddH₂O as a negative control. Marker lane, DL-2000. LAMP, loop-mediated isothermal amplification; cdt, Clostridium difficile transferase; PCR, polymerase chain reaction.