miR-146b-5p directly targets TRAF6. (A) Interaction between miR-146b-5p and the 3′UTR of TRAF6 was predicted using TargetScan and MiRanda databases. (B) Luciferase activity of cells transfected with a reporter vector containing a WT or mutant TRAF6 3′UTR together with miR-146b-5p or miR-C are presented in the bar graph. **P<0.01 vs. control. TRAF6 3′UTR-MUT, TRAF6 3′UTR with a mutation in the miR-146b-5p binding site; UTR, untranslated region; WT, wild-type; TRAF6, tumor necrosis factor receptor-associated factor 6; miR, microRNA; miR-C, control miR; hsa, Homo sapiens.