Figure 5.

Effect of CHAF1A silencing on cell cycle progression in H1299 cells. (A and B) Cells transduced with control shRNA- or CHAF1A shRNA-expressing lentivirus were stained with PI and assessed by flow cytometry. (A) Representative histograms of cell cycle distribution. (B) Quantification of the percentage of cells at each cell cycle phase. (C) Western blot analysis of indicated proteins. Bar graphs indicate densitometric analysis of protein signals from three independent experiments. *P<0.05 vs. Control shRNA. Data are representative of three experiments performed in triplicates (n=9). Data are presented as mean ± standard deviation. PI, propidium iodide; CHAF1A, chromatin assembly factor 1 subunit A; shRNA, short hairpin RNA; CDK, cyclin dependent kinase; SKP, S-phase kinase associated protein.