Figure 1.

The internalization and not the auxin inhibition of internalization of the transgenically expressed PIN2-GFP is impaired in gsnor1-3 mutant. Six-day-old ProPIN2:PIN2-GFP-expressing transgenic seedlings either in Col-0 or in gsnor1-3 background were treated with Mock (A,D), 50 μM BFA for 60 min (B,E), and with 10 μM 2, 4-D for 30 min, followed by treatment with 50 μM BFA+ 10 μM 2, 4-D for additional 60 min (C,F). Images were captured by confocal laser scanning microscopy (CLSM, Leica TCS SP5 AOBS). The numbers of BFA bodies (see white arrows) were counted and the fluorescence intensities both at the BFA bodies and at the PM were measured, respectively, using Image J (http://rsb.info.nih.gov/ij) and the statistical data were summarized (G–J). (G) GFP intensity at the PM; (H) Number of BFA bodies per cell; (I) Relative GFP intensities of the BFA bodies; (J) The GFP intensity ratios of the BFA bodies/PM. Bar = 50 μm. ^***Indicates significant differences between Col-0 and gsnor1-3 by Student's t-test at 0.001 level.