Figure 4.

Exogenously applied GSNO reduces both the internalization and the PM-associated level of PIN2-GFP in the Col-0 seedlings in a concentration-dependent manner. The 2-day-old wild type Col-0 seedlings grown on ½ MS agar plates were transferred onto the agar plates containing different concentration of GSNO (A–C), or different concentration of GSNO plus 50 μM BFA (E–G) for additional 4 day. The gsnor1-3 mutant is used as a control (D,H). The images were captured by confocal laser scanning microscopy (CLSM, Leica TCS SP5 AOBS). The numbers of BFA bodies (see white arrows) were counted (I) and the fluorescence intensities of the BFA bodies and at the PM were measured, respectively, using Image J (http://rsb.info.nih.gov/ij). The GFP intensity ratios of BFA bodies to PM were measured and the statistical data were presented in (J). Bar = 50 μm. a, b, c, and d indicate significant differences between the treatments by Student's t-test.