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. 2017 Nov 28;23:46. doi: 10.1186/s40409-017-0136-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — RP-HPLC profile of the crude Cdt venom. F1 to F6 correspond to the manually collected fractions. F1 and F2: crotamin; F3: crotapotin; F4, F5 and F6: PLA₂. UV monitoring 214 nm. Inset: F3 analytical RP-HPLC demonstrating the proper molecule isolation. Chromatographic conditions are described in Methods section