Fig. 2.

qRT-PCR analyses of gene expression levels in AqPI-VIGS tissue. a Expression of AqPI in first whorl organs (W1), second whorl organs (W2), stamens transformed into sepals (STA to SEP), stamens transformed into petals (STA to PET), and stamens transformed into carpels (STA to CAR). All values are normalized to the expression of AqPI in control AqANS-VIGS samples for each organ type (dotted line). b Expression of the three Aquilegia AP3 homologs in same samples as a. All values are normalized to the expression levels of each gene in control samples for each organ type (dotted line). c Expression of AqAG1 in stamens transformed to sepals (STA to SEP), stamens to petals (STA to PET), and stamens to carpels (STA to CAR). All values are normalized to the expression of AqAG1 in control AqANS-VIGS stamens (dotted line). d Expression of AqPI, AqANS, AqF3H, and AqDFR across two classes of sepal tissue: AqANS-VIGS control tissue and AqPI-VIGS tissue. All values are normalized relative to the expression of each gene in untreated sepals (dotted line). All vertical axes represent relative expression levels, all asterisks indicate expression levels that are significantly different from the reference control organs, and all error bars represent standard deviation